It has been held the retrospective analysis of 332 health histories, who were treated at the 22 of the Department Pediatric Hospital, which was isolated Acinetobacter spp. Cases of the mole and cases of the intrahospital infection were registered in 20 of the Department. In just 5 years it has been registered 332 cases of Acinetobacter spp., including 218 cases of the mole and 114 cases of the intrahospital infection. For 5 years the medial level value of the intrahospital acinetobacteria infection incidence reached to 0,63 %. The spectrum of the marked Acinetobacter spp. in mole the Acinetobacter lwiffii has been prevailed, its specific gravity reached up to 54 %, Acinetobacter baumannii – 34 %, other (Acinetobacter anitratus, Acinetobacter junii) – in single cases. In intrahospital cases it was Acinetobacter baumannii, which specific gravity reached up to 50 % and Acinetobacter lwoffii – 46 %, others species occur as well in single cases. Antibiotic sensitivity was studied to 26 antimicrobial preparations. From a study of 114 strains isolated during nosocomial infection, 15 strains were highly resistant to most drugs studied, 45 – moderately sensitive and only 54 – sensitive to all drugs.